Activation and Differentiation of Mesenchymal Stem Cells.
Methods Mol Biol
; 1554: 201-209, 2017.
Article
em En
| MEDLINE
| ID: mdl-28185193
ABSTRACT
Mesenchymal stem cells (MSCs) are multipotent cells and exhibit two main characteristics that define stem cells self-renewal and differentiation. MSCs can migrate to sites of injury, inflammation, and tumor. Moreover, MSCs undergo myofibroblast like differentiation, including increased production of α-SMA in response to transforming growth factor-ß (TGF-ß), a growth factor commonly secreted by tumor cells to evade immune surveillance. Based on our previous finding hMSCs become activated and resemble carcinoma-associated myofibroblasts upon prolonged exposure to conditioned medium from MDAMB231 human breast cancer cells. Here, we show that keratinocyte conditioned medium (KCM) induces differentiation of MSCs to resemble dermal myofibroblast like cells using immunofluorescence techniques demonstrating punctate vinculin staining, and F-actin filaments.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Diferenciação Celular
/
Células-Tronco Mesenquimais
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article