Insights into the formation of carlactone from in-depth analysis of the CCD8-catalyzed reactions.
FEBS Lett
; 591(5): 792-800, 2017 03.
Article
em En
| MEDLINE
| ID: mdl-28186640
ABSTRACT
Strigolactones are a new class of phytohormones synthesized from carotenoids via carlactone. The complex structure of carlactone is not easily deducible from its precursor, a cis-configured ß-carotene cleavage product, and is thus formed via a poorly understood series of reactions and molecular rearrangements, all catalyzed by only one enzyme, the carotenoid cleavage dioxygenase 8 (CCD8). Moreover, the reactions leading to carlactone are expected to form a second, yet unidentified product. In this study, we used 13 C and 18 O-labeling to shed light on the reactions catalyzed by CCD8. The characterization of the resulting carlactone by LC-MS and NMR, and the identification of the assumed, less accessible second product allowed us to formulate a minimal reaction mechanism for carlactone generation.
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MEDLINE
Assunto principal:
Reguladores de Crescimento de Plantas
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Proteínas de Plantas
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Carotenoides
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Beta Caroteno
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Dioxigenases
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Lactonas
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article