Evaluation of a hydrophilic interaction liquid chromatography design space for sugars and sugar alcohols.
J Chromatogr A
; 1489: 65-74, 2017 Mar 17.
Article
em En
| MEDLINE
| ID: mdl-28190593
ABSTRACT
Based on a column-screening exercise, a column ranking system was developed for sample mixtures containing any combination of 26 sugar and sugar alcohol analytes using 16 polar stationary phases in the HILIC mode with acetonitrile/water or acetone/water mobile phases. Each analyte was evaluated on the HILIC columns with gradient elution and the subsequent chromatography data was compiled into a statistical software package where any subset of the analytes can be selected and the columns are then ranked by the greatest separation. Since these analytes lack chromophores, aerosol-based detectors, including an evaporative light scattering detector (ELSD) and a charged aerosol detector (CAD) were employed for qualitative and quantitative detection. Example qualitative applications are provided to illustrate the practicality and efficiency of this HILIC column ranking. Furthermore, the design-space approach was used as a starting point for a quantitative method for the trace analysis of glucose in trehalose samples in a complex matrix. Knowledge gained from evaluating the design-space led to rapid development of a capable method as demonstrated through validation of the following parameters specificity, accuracy, precision, linearity, limit of quantitation, limit of detection, and range.
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Base de dados:
MEDLINE
Assunto principal:
Álcoois Açúcares
/
Carboidratos
/
Cromatografia Líquida de Alta Pressão
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article