Your browser doesn't support javascript.
loading
Automated Trapping Column Exchanger for High-Throughput Nanoflow Liquid Chromatography.
Spencer, Sandra E; Corso, Thomas N; Bollinger, James G; Henderson, Clark M; Hoofnagle, Andrew N; MacCoss, Michael J.
Afiliação
  • Spencer SE; Department of Genome Sciences, University of Washington School of Medicine , Box 355065, Seattle, Washington 98195-5065, United States.
  • Corso TN; CorSolutions, LLC. , Cornell Business and Technology Park, 95 Brown Road, Box 1007, Ithaca, New York 14850-1294, United States.
  • Bollinger JG; Department of Genome Sciences, University of Washington School of Medicine , Box 355065, Seattle, Washington 98195-5065, United States.
  • Henderson CM; Department of Laboratory Medicine, University of Washington School of Medicine , Box 357110, Seattle, Washington 98195-7110, United States.
  • Hoofnagle AN; Department of Laboratory Medicine, University of Washington School of Medicine , Box 357110, Seattle, Washington 98195-7110, United States.
  • MacCoss MJ; Department of Genome Sciences, University of Washington School of Medicine , Box 355065, Seattle, Washington 98195-5065, United States.
Anal Chem ; 89(4): 2383-2389, 2017 02 21.
Article em En | MEDLINE | ID: mdl-28192907
As compared to conventional high-performance liquid chromatography (HPLC) techniques, nanoflow HPLC exhibits improved sensitivity and limits of detection. However, nanoflow HPLC suffers from low throughput due to instrument failure (e.g., fitting fatigue and trapping column failure), limiting the utility of the technique for clinical and industrial applications. To increase the robustness of nanoflow HPLC, we have developed and tested a trapping column exchanging robot for autonomous interchange of trapping columns. This robot makes reproducible, automated connections between the active trapping column and the rest of the HPLC system. The intertrapping column retention time is shown to be sufficiently reproducible for scheduled selected reaction monitoring assays to be performed on different trapping columns without rescheduling the selection windows.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Cromatografia Líquida de Alta Pressão Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Cromatografia Líquida de Alta Pressão Idioma: En Ano de publicação: 2017 Tipo de documento: Article