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Regulation of the wheat MAP kinase phosphatase 1 by 14-3-3 proteins.
Ghorbel, Mouna; Cotelle, Valérie; Ebel, Chantal; Zaidi, Ikram; Ormancey, Mélanie; Galaud, Jean-Philippe; Hanin, Moez.
Afiliação
  • Ghorbel M; Laboratory of Biotechnology and Plant Improvement, Center of Biotechnology of Sfax, BP1177, 3018 Sfax, Tunisia; Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, 24, chemin de Borde-Rouge, BP 42617, 31326 Castanet-Tolosan, France.
  • Cotelle V; Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, 24, chemin de Borde-Rouge, BP 42617, 31326 Castanet-Tolosan, France.
  • Ebel C; Laboratory of Biotechnology and Plant Improvement, Center of Biotechnology of Sfax, BP1177, 3018 Sfax, Tunisia; University of Sfax, Institute of Biotechnology, BP "1175", 3038 Sfax, Tunisia.
  • Zaidi I; Laboratory of Biotechnology and Plant Improvement, Center of Biotechnology of Sfax, BP1177, 3018 Sfax, Tunisia.
  • Ormancey M; Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, 24, chemin de Borde-Rouge, BP 42617, 31326 Castanet-Tolosan, France.
  • Galaud JP; Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, 24, chemin de Borde-Rouge, BP 42617, 31326 Castanet-Tolosan, France. Electronic address: galaud@lrsv.ups-tlse.fr.
  • Hanin M; Laboratory of Biotechnology and Plant Improvement, Center of Biotechnology of Sfax, BP1177, 3018 Sfax, Tunisia; University of Sfax, Institute of Biotechnology, BP "1175", 3038 Sfax, Tunisia. Electronic address: moez.hanin@cbs.rnrt.tn.
Plant Sci ; 257: 37-47, 2017 Apr.
Article em En | MEDLINE | ID: mdl-28224917
ABSTRACT
Plant MAP kinase phosphatases (MKPs) are major regulators of MAPK signaling pathways and play crucial roles in controlling growth, development and stress responses. The presence of several functional domains in plant MKPs such as a dual specificity phosphatase catalytic domain, gelsolin, calmodulin-binding and serine-rich domains, suggests that MKPs can interact with distinct cellular partners, others than MAPKs. In this report, we identified a canonical mode I 14-3-3-binding motif (574KLPSLP579) located at the carboxy-terminal region of the wheat MKP, TMKP1. We found that this motif is well-conserved among other MKPs from monocots including Hordeum vulgare, Brachypodium distachyon and Aegilops taushii. Using co-immunoprecipitation assays, we provide evidence for interaction between TMKP1 and 14-3-3 proteins in wheat. Moreover, the phosphatase activity of TMKP1 is increased in a phospho-dependent manner by either Arabidopsis or yeast 14-3-3 isoforms. TMKP1 activation by 14-3-3 proteins is enhanced by Mn2+, whereas in the presence of Ca2+ ions, TMKP1 activation was limited to Arabidopsis 14-3-3φ (phi), an isoform harboring an EF-hand motif. Such findings strongly suggest that 14-3-3 proteins, in conjunction with specific divalent cations, may stimulate TMKP1 activity and point-out that 14-3-3 proteins bind and regulate the activity of a MKP in eukaryotes.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Triticum / Proteínas 14-3-3 / Fosfatase 1 de Especificidade Dupla Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Triticum / Proteínas 14-3-3 / Fosfatase 1 de Especificidade Dupla Idioma: En Ano de publicação: 2017 Tipo de documento: Article