Expression profiles of eNOS, iNOS and microRNA-27b in the corpus cavernosum of rats submitted to chronic alcoholism and Diabetes mellitus.
Acta Cir Bras
; 32(1): 38-45, 2017 Jan.
Article
em En
| MEDLINE
| ID: mdl-28225916
ABSTRACT
PURPOSE:
To evaluate the expression of endothelial and inducible NOS in addition to the miRNA-27b in the corpus cavernosum and peripheral blood of healthy rats, diabetic rats, alcoholic rats and rats with both pathologies.METHODS:
Forty eight Wistar rats were divided into four groups control (C), alcoholic (A), diabetic (D) and alcoholic-diabetic (AD). Samples of the corpus cavernosum were prepared to study protein expressions of eNOS and iNOS by immunohistochemistry and expression of miRNA-27b in the corpus cavernosum and peripheral blood.RESULTS:
Immunohistochemistry for eNOS and iNOS showed an increase in cavernosal smooth muscle cells in the alcoholic, diabetic and alcoholic-diabetic groups when compared with the control group. Similarly, the mRNA levels for eNOS were increased in cavernosal smooth muscle (CSM) in the alcoholic, diabetic and alcoholic-diabetic groups and miRNA-27b were decreased in CSM in the alcoholic, diabetic and alcoholic-diabetic groups.CONCLUSION:
The major new finding of our study was an impairment of relaxation of cavernosal smooth muscle in alcoholic, diabetic, and alcoholic-diabetic rats that involved a decrease in the nitric oxide pathway by endothelium-dependent mechanisms accompanied by a change in the corpus cavernosum contractile sensitivity.
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Base de dados:
MEDLINE
Assunto principal:
Pênis
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MicroRNAs
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Diabetes Mellitus Experimental
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Alcoolismo
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Óxido Nítrico Sintase Tipo II
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Óxido Nítrico Sintase Tipo III
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article