Your browser doesn't support javascript.
loading
Expression profiles of eNOS, iNOS and microRNA-27b in the corpus cavernosum of rats submitted to chronic alcoholism and Diabetes mellitus.
Cunha, Joao Paulo da; Lizarte, Fermino Sanches; Novais, Paulo Cezar; Gattas, Daniela; Carvalho, Camila Albuquerque Mello de; Tirapelli, Daniela Pretti da Cunha; Molina, Carlos Augusto Fernandes; Tirapelli, Luis Fernando; Tucci, Silvio.
Afiliação
  • Cunha JP; Master, Postgraduate Program in Clinical Surgery, Department of Surgery and Anatomy, School of Medicine of Ribeirao Preto, Universidade de São Paulo (USP), Ribeirao Preto-SP, Brazil. Acquisition and interpretation of data, manuscript writing.
  • Lizarte FS; Pos-doctoral Fellow student, Postgraduate Program in Clinical Surgery, Department of Surgery and Anatomy, School of Medicine of Ribeirao Preto, USP, Ribeirao Preto-SP, Brazil. Technical procedures, manuscript writing.
  • Novais PC; Assistant Professor, Department of Health Sciences, Universidade de Marília, Brazil. Technical procedures, manuscript writing.
  • Gattas D; Fellow Master degree, Postgraduate Program in Clinical Surgery, Department of Surgery and Anatomy, School of Medicine of Ribeirao Preto, USP, Ribeirao Preto-SP, Brazil. Manuscript writing.
  • Carvalho CA; Assistant Professor, Institute of Biological and Health Sciences, Federal University of Alagoas, Alagoas, Brazil and PhD Student, Clinical Surgery Postgraduate Program, Department of Surgery and Anatomy, School of Medicine of Ribeirao Preto, USP, Ribeirao Preto-SP, Brazil. Manuscript writing.
  • Tirapelli DP; Assistant Professor, Department of Surgery and Anatomy, Surgical Clinic Program, School of Medicine of Ribeirao Preto, USP, Ribeirao Preto-SP, Brazil, Scientific and intellectual content of the study.
  • Molina CA; Assistant Professor, Division of Urology, Department of Surgery and Anatomy, School of Medicine of Ribeirao Preto, USP, Ribeirao Preto-SP, Brazil. Scientific and intellectual content of the study.
  • Tirapelli LF; Associate Professor, Department of Surgery and Anatomy, School of Medicine of Ribeirao Preto, USP, Ribeirao Preto-SP, Brazil. Scientific and intellectual content of the study.
  • Tucci S; Associate Professor, Division of Urology, Department of Surgery and Anatomy, School of Medicine of Ribeirao Preto, USP, Ribeirao Preto-SP, Brazil. Concept, design, intellectual and scientific content of the study; critical revision; supervised all phases of the study.
Acta Cir Bras ; 32(1): 38-45, 2017 Jan.
Article em En | MEDLINE | ID: mdl-28225916
ABSTRACT

PURPOSE:

To evaluate the expression of endothelial and inducible NOS in addition to the miRNA-27b in the corpus cavernosum and peripheral blood of healthy rats, diabetic rats, alcoholic rats and rats with both pathologies.

METHODS:

Forty eight Wistar rats were divided into four groups control (C), alcoholic (A), diabetic (D) and alcoholic-diabetic (AD). Samples of the corpus cavernosum were prepared to study protein expressions of eNOS and iNOS by immunohistochemistry and expression of miRNA-27b in the corpus cavernosum and peripheral blood.

RESULTS:

Immunohistochemistry for eNOS and iNOS showed an increase in cavernosal smooth muscle cells in the alcoholic, diabetic and alcoholic-diabetic groups when compared with the control group. Similarly, the mRNA levels for eNOS were increased in cavernosal smooth muscle (CSM) in the alcoholic, diabetic and alcoholic-diabetic groups and miRNA-27b were decreased in CSM in the alcoholic, diabetic and alcoholic-diabetic groups.

CONCLUSION:

The major new finding of our study was an impairment of relaxation of cavernosal smooth muscle in alcoholic, diabetic, and alcoholic-diabetic rats that involved a decrease in the nitric oxide pathway by endothelium-dependent mechanisms accompanied by a change in the corpus cavernosum contractile sensitivity.
Assuntos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Pênis / MicroRNAs / Diabetes Mellitus Experimental / Alcoolismo / Óxido Nítrico Sintase Tipo II / Óxido Nítrico Sintase Tipo III Idioma: En Ano de publicação: 2017 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Pênis / MicroRNAs / Diabetes Mellitus Experimental / Alcoolismo / Óxido Nítrico Sintase Tipo II / Óxido Nítrico Sintase Tipo III Idioma: En Ano de publicação: 2017 Tipo de documento: Article