Phase-contrast MR flow imaging: A tool to determine hepatic hemodynamics in rats with a healthy, fibrotic, or cirrhotic liver.
J Magn Reson Imaging
; 46(5): 1526-1534, 2017 11.
Article
em En
| MEDLINE
| ID: mdl-28240794
ABSTRACT
PURPOSE:
To test a magnetic resonance (MR) scanning protocol as a noninvasive tool to determine hepatic hemodynamics and to assess the degree of liver fibrosis in an animal model of liver fibrosis and cirrhosis. MATERIALS ANDMETHODS:
Fifty-four male Wistar rats were studied. Thirty-nine received thioacetamide (TAA) in their drinking water for either 12 or 16 weeks. MR measurements were performed using flow-sensitive 2D phase-contrast MRI and a 9.4T preclinical scanner. The following hemodynamic parameters were investigated portal cross-sectional area, mean portal flow velocity, and portal and aortic flow volume rate. Therefore, rats (n = 46) were divided into three groups CON (control, n = 13), FIB (fibrosis, n = 25), and CIR (cirrhosis, n = 8). Furthermore, the degree of liver fibrosis was assessed by a self-established MR score and verified by a standardized histological score (n = 48).RESULTS:
Portal and aortic flow parameters could be reliably detected. A significant decrease in portal flow velocity was found in FIB (FIB vs. CON -21%, P = 0.006 and CIR vs. CON -17%, P = 0.105) and in portal flow volume rate in FIB and CIR (FIB vs. CON -20%, P = 0.009 and CIR vs. CON -25%, P = 0.024). If the histological score is taken as standard, the self-established MR score enabled discrimination between healthy and diseased livers (sensitivity to identify diseased livers 89% and specificity to identify healthy livers 100%).CONCLUSION:
This MR scanning protocol presents a noninvasive tool to determine hepatic hemodynamics in healthy and diseased rats. LEVEL OF EVIDENCE 2 Technical Efficacy Stage 1 J. Magn. Reson. Imaging 2017;461526-1534.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Imageamento por Ressonância Magnética
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Fígado
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Cirrose Hepática
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article