Exosomes function in antigen presentation during an in vivo Mycobacterium tuberculosis infection.
Sci Rep
; 7: 43578, 2017 03 06.
Article
em En
| MEDLINE
| ID: mdl-28262829
ABSTRACT
Mycobacterium tuberculosis-infected macrophages and dendritic cells are limited in their ability to present antigen to CD4+ T cells suggesting that other mechanism of antigen presentation are driving the robust T cell response observed during an M. tuberculosis infection. These mechanisms could include antigens present in apoptotic bodies, necrotic debris, exosomes or even release of non-vesicular antigen from infected cells. However, there is limited data to support any of these mechanisms as important in driving T cell activation in vivo. In the present study we use Rab27a-deficient mice which show diminished trafficking of mycobacterial components to exosomes as well as M. tuberculosis strains that express recombinant proteins which traffic or fail to traffic to exosomes. We observed that exosomes released during a mouse M. tuberculosis infection contribute significantly to its T cell response. These finding imply that exosomes function to promote T cell immunity during a bacterial infection and are an important source of extracellular antigen.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Tuberculose
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Apresentação de Antígeno
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Exossomos
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Mycobacterium tuberculosis
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Antígenos de Bactérias
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article