A key role of nuclear factor Y in the refeeding response of fatty acid synthase in adipocytes.
FEBS Lett
; 591(7): 965-978, 2017 Apr.
Article
em En
| MEDLINE
| ID: mdl-28281280
ABSTRACT
Fatty acid synthase (Fasn) is a key component of energy metabolism that is dynamically induced by food intake. Although extensive studies have revealed a number of transcription factors involved in the fasting/refeeding transition of Fasn expression in hepatocytes, much less evidence is available for adipocytes. Using the in vivo Ad-luc analytical system, we identified the inverted CCAAT element (ICE) around -100 nucleotides in the Fasn promoter as a critical cis-element for the refeeding response in adipocytes. Electrophoretic mobility shift assays and chromatin immunoprecipitation show that nuclear factor Y (NF-Y) binds to ICE specifically in refeeding states. Notably, the NF-Y binding to ICE is differently regulated between adipocytes and hepatocytes. These findings provide insights into the specific mechanisms controlling energy metabolism in adipocytes.
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Base de dados:
MEDLINE
Assunto principal:
Adipócitos
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Fator de Ligação a CCAAT
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Ácido Graxo Sintases
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Comportamento Alimentar
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article