Culturing human intestinal stem cells for regenerative applications in the treatment of inflammatory bowel disease.
EMBO Mol Med
; 9(5): 558-570, 2017 05.
Article
em En
| MEDLINE
| ID: mdl-28283650
ABSTRACT
Both the incidence and prevalence of inflammatory bowel disease (IBD) is increasing globally; in the industrialized world up to 0.5% of the population are affected and around 4.2 million individuals suffer from IBD in Europe and North America combined. Successful engraftment in experimental colitis models suggests that intestinal stem cell transplantation could constitute a novel treatment strategy to re-establish mucosal barrier function in patients with severe disease. Intestinal stem cells can be grown in vitro in organoid structures, though only a fraction of the cells contained are stem cells with regenerative capabilities. Hence, techniques to enrich stem cell populations are being pursued through the development of multiple two-dimensional and three-dimensional culture protocols, as well as co-culture techniques and multiple growth medium compositions. Moreover, research in support matrices allowing for efficient clinical application is in progress. In vitro culture is accomplished by modulating the signaling pathways fundamental for the stem cell niche with a suitable culture matrix to provide additional contact-dependent stimuli and structural support. The aim of this review was to discuss medium compositions and support matrices for optimal intestinal stem cell culture, as well as potential modifications to advance clinical use in IBD.
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Base de dados:
MEDLINE
Assunto principal:
Células-Tronco
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Doenças Inflamatórias Intestinais
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Técnicas de Cultura de Células
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Meios de Cultura
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Transplante de Células-Tronco
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Alicerces Teciduais
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Intestinos
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article