Development of RAP Tag, a Novel Tagging System for Protein Detection and Purification.
Monoclon Antib Immunodiagn Immunother
; 36(2): 68-71, 2017 Apr.
Article
em En
| MEDLINE
| ID: mdl-28339303
ABSTRACT
Affinity tag systems, possessing high affinity and specificity, are useful for protein detection and purification. The most suitable tag for a particular purpose should be selected from many available affinity tag systems. In this study, we developed a novel affinity tag called the "RAP tag" system, which comprises a mouse antirat podoplanin monoclonal antibody (clone PMab-2) and the RAP tag (DMVNPGLEDRIE). This system is useful not only for protein detection in Western blotting, flow cytometry, and sandwich enzyme-linked immunosorbent assay, but also for protein purification.
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MEDLINE
Assunto principal:
Peptídeos
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Coloração e Rotulagem
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Glicoproteínas de Membrana
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Receptores ErbB
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Anticorpos Monoclonais
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article