Reduced expression of adipose triglyceride lipase decreases arachidonic acid release and prostacyclin secretion in human aortic endothelial cells.
Arch Physiol Biochem
; 123(4): 249-253, 2017 Oct.
Article
em En
| MEDLINE
| ID: mdl-28368219
ABSTRACT
BACKGROUND:
Vascular endothelial cells represent an important source of arachidonic acid (AA)-derived mediators involved in the generation of anti- or proatherogenic environments. Evidence emerged (in mast cells), that in addition to phospholipases, neutral lipid hydrolases as adipose triglyceride lipase (ATGL) also participate in this process.OBJECTIVE:
To examine the impact of ATGL on AA-release from cellular phospholipids (PL) and on prostacyclin secretion in human aortic endothelial cells (HAEC). METHODS ANDRESULTS:
siRNA-mediated silencing of ATGL promoted lipid droplet formation and TG accumulation in HAEC (nile red stain). ATGL knockdown decreased the basal and A23187 (calcium ionophore)-induced release of 14C-AA from (14C-AA-labeled) HAEC. In A23187-stimulated ATGL silenced cells, this was accompanied by a decreased content of 14C-AA in cellular PL and a decreased secretion of prostacyclin (determined by 6-keto PGF1α EIA).CONCLUSIONS:
In vascular endothelial cells, the efficiency of stimulus-induced AA release and prostacyclin secretion is dependent on ATGL.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Aorta
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Endotélio Vascular
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Epoprostenol
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Ácido Araquidônico
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Lipase
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article