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Modeling of Dolichol Mass Spectra Isotopic Envelopes as a Tool to Monitor Isoprenoid Biosynthesis.
Jozwiak, Adam; Lipko, Agata; Kania, Magdalena; Danikiewicz, Witold; Surmacz, Liliana; Witek, Agnieszka; Wojcik, Jacek; Zdanowski, Konrad; Paczkowski, Cezary; Chojnacki, Tadeusz; Poznanski, Jaroslaw; Swiezewska, Ewa.
Afiliação
  • Jozwiak A; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland (A.J., A.L., L.S., A.W., J.W., K.Z., T.C., J.P., E.S.).
  • Lipko A; Institute of Organic Chemistry, Polish Academy of Sciences, 01-224 Warsaw, Poland (M.K., W.D.).
  • Kania M; Institute of Chemistry, University of Natural Sciences and Humanities, 08-110 Siedlce, Poland (K.Z.); and.
  • Danikiewicz W; Department of Plant Biochemistry, Faculty of Biology, University of Warsaw, 02-096 Warsaw, Poland (C.P.).
  • Surmacz L; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland (A.J., A.L., L.S., A.W., J.W., K.Z., T.C., J.P., E.S.).
  • Witek A; Institute of Organic Chemistry, Polish Academy of Sciences, 01-224 Warsaw, Poland (M.K., W.D.).
  • Wojcik J; Institute of Chemistry, University of Natural Sciences and Humanities, 08-110 Siedlce, Poland (K.Z.); and.
  • Zdanowski K; Department of Plant Biochemistry, Faculty of Biology, University of Warsaw, 02-096 Warsaw, Poland (C.P.).
  • Paczkowski C; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland (A.J., A.L., L.S., A.W., J.W., K.Z., T.C., J.P., E.S.).
  • Chojnacki T; Institute of Organic Chemistry, Polish Academy of Sciences, 01-224 Warsaw, Poland (M.K., W.D.).
  • Poznanski J; Institute of Chemistry, University of Natural Sciences and Humanities, 08-110 Siedlce, Poland (K.Z.); and.
  • Swiezewska E; Department of Plant Biochemistry, Faculty of Biology, University of Warsaw, 02-096 Warsaw, Poland (C.P.).
Plant Physiol ; 174(2): 857-874, 2017 Jun.
Article em En | MEDLINE | ID: mdl-28385729
ABSTRACT
The cooperation of the mevalonate (MVA) and methylerythritol phosphate (MEP) pathways, operating in parallel in plants to generate isoprenoid precursors, has been studied extensively. Elucidation of the isoprenoid metabolic pathways is indispensable for the rational design of plant and microbial systems for the production of industrially valuable terpenoids. Here, we describe a new method, based on numerical modeling of mass spectra of metabolically labeled dolichols (Dols), designed to quantitatively follow the cooperation of MVA and MEP reprogrammed upon osmotic stress (sorbitol treatment) in Arabidopsis (Arabidopsis thaliana). The contribution of the MEP pathway increased significantly (reaching 100%) exclusively for the dominating Dols, while for long-chain Dols, the relative input of the MEP and MVA pathways remained unchanged, suggesting divergent sites of synthesis for dominating and long-chain Dols. The analysis of numerically modeled Dol mass spectra is a novel method to follow modulation of the concomitant activity of isoprenoid-generating pathways in plant cells; additionally, it suggests an exchange of isoprenoid intermediates between plastids and peroxisomes.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Terpenos / Arabidopsis / Espectrometria de Massas por Ionização por Electrospray / Dolicóis / Modelos Teóricos Idioma: En Ano de publicação: 2017 Tipo de documento: Article
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Terpenos / Arabidopsis / Espectrometria de Massas por Ionização por Electrospray / Dolicóis / Modelos Teóricos Idioma: En Ano de publicação: 2017 Tipo de documento: Article