Gene and protein expression of a soluble form of CTLA-4 in a healthy dog.
J Vet Med Sci
; 79(5): 871-875, 2017 May 18.
Article
em En
| MEDLINE
| ID: mdl-28392507
ABSTRACT
Cytotoxic T lymphocyte associated gene-4 (CTLA-4) is a costimulatory molecule, expressed on the surface of activated T cells that negatively regulates T cell activation. In humans, alternative splicing of the CTLA-4 gene generates two major isoforms of mRNA, and a soluble form of CTLA-4 (sCTLA-4) was detected in normal human serum. We describe alternatively spliced mRNA expressed in peripheral blood mononuclear cells obtained from a healthy dog lacking the transmembrane domain coded by exon 3 of the CTLA-4 gene. Immunoprecipitation and western blotting of dog serum revealed a band of approximately 23-kDa, which is consistent with the predicted size, based on the amino acid sequence of the canine sCTLA-4 obtained in this study.
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MEDLINE
Assunto principal:
Cães
/
Antígeno CTLA-4
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article