Threshold value of LIAISON XL anti-HCV screening assay predicting positive immunoblotting results.
J Med Virol
; 89(10): 1817-1822, 2017 10.
Article
em En
| MEDLINE
| ID: mdl-28401710
ABSTRACT
Some international guidelines recommend evaluating the need to confirm positive anti-hepatitis C virus (HCV) antibody screening results by means of a more specific antibody or molecular biology test on the basis of a screening threshold value (such as the sample signal/cut-off ratio) that can predict the positivity of additional antibody testing in at least 95% of cases. The aim of this study was to determine the threshold value of the DiaSorin LIAISON XL chemiluminescence test. Two hundred and twenty-eight routine laboratory samples that were chemiluminescence positive for anti-HCV antibodies but had different signal/cut-off ratios were assayed using immunoblotting, which indicated that 155 (68.0%) were positive, 40 (17.5%) were negative, and 33 (14.5%) were indeterminate. When the samples were divided on the basis of their signal/cut-off ratios, 95.5% of the samples with a ratio of ≥3.5 were positive as against 74.1% of the positive or indeterminate samples with a ratio of <3.5. Statistical analysis using Youden's index and a receiver operating characteristic curve showed that the optimum cut-off value was 3.65. These findings indicate that, when using the LIAISON XL system for anti-HCV antibody screening, a signal/cut-off ratio of ≥3.65 makes further confirmatory tests unnecessary.
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Base de dados:
MEDLINE
Assunto principal:
Doadores de Sangue
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Imunoensaio
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Immunoblotting
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Hepatite C
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Anticorpos Anti-Hepatite C
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Medições Luminescentes
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article