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Characterization the carotenoid productions and profiles of three Rhodosporidium toruloides mutants from Agrobacterium tumefaciens-mediated transformation.
Lin, Xinping; Gao, Ning; Liu, Sasa; Zhang, Sufang; Song, Shuang; Ji, Chaofan; Dong, Xiuping; Su, Yichen; Zhao, Zongbao Kent; Zhu, Beiwei.
Afiliação
  • Lin X; School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian, 116034, People's Republic of China.
  • Gao N; School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian, 116034, People's Republic of China.
  • Liu S; School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian, 116034, People's Republic of China.
  • Zhang S; Division of Biotechnology, Dalian Institute of Chemical Physics, Dalian, 116023, People's Republic of China.
  • Song S; School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian, 116034, People's Republic of China.
  • Ji C; School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian, 116034, People's Republic of China.
  • Dong X; School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian, 116034, People's Republic of China.
  • Su Y; Seafood Research and Education Center, Oregon State University, Astoria, Oregon, 97103, USA.
  • Zhao ZK; Division of Biotechnology, Dalian Institute of Chemical Physics, Dalian, 116023, People's Republic of China.
  • Zhu B; School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian, 116034, People's Republic of China.
Yeast ; 34(8): 335-342, 2017 08.
Article em En | MEDLINE | ID: mdl-28426167
ABSTRACT
The red yeast Rhodosporidium toruloides is a known lipid producer capable of accumulating large amounts of triacylglycerols and carotenoids. However, it remains challenging to study its carotenoid production profiles owing to limited biochemical information and inefficient genetic tools. Here we used an Agrobacterium tumefaciens-mediated transformation (ATMT) to change its carotenoid production and profiles. We constructed R. toruloides NP11 mutant libraries with ATMT, selected three mutants with different colours, characterized their carotenoid products by high-pressure liquid chromatography-mass spectrometry (HPLC-MS) analysis and assured differences among those strains in terms of carotenoid production and its composition profiles. We then located T-DNA insertion sites using the genome walking technology and provided discussions in terms of the new phenotypes. This study is the first of its kind to change the carotenoid production profiles in R. toruloides. Copyright © 2017 John Wiley & Sons, Ltd.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Rhodotorula / Transformação Genética / Carotenoides / Redes e Vias Metabólicas / Engenharia Metabólica Idioma: En Ano de publicação: 2017 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Rhodotorula / Transformação Genética / Carotenoides / Redes e Vias Metabólicas / Engenharia Metabólica Idioma: En Ano de publicação: 2017 Tipo de documento: Article