Mural lymphatic endothelial cells regulate meningeal angiogenesis in the zebrafish.
Nat Neurosci
; 20(6): 774-783, 2017 Jun.
Article
em En
| MEDLINE
| ID: mdl-28459441
ABSTRACT
Mural cells of the vertebrate brain maintain vascular integrity and function, play roles in stroke and are involved in maintenance of neural stem cells. However, the origins, diversity and roles of mural cells remain to be fully understood. Using transgenic zebrafish, we identified a population of isolated mural lymphatic endothelial cells surrounding meningeal blood vessels. These meningeal mural lymphatic endothelial cells (muLECs) express lymphatic endothelial cell markers and form by sprouting from blood vessels. In larvae, muLECs develop from a lymphatic endothelial loop in the midbrain into a dispersed, nonlumenized mural lineage. muLEC development requires normal signaling through the Vegfc-Vegfd-Ccbe1-Vegfr3 pathway. Mature muLECs produce vascular growth factors and accumulate low-density lipoproteins from the bloodstream. We find that muLECs are essential for normal meningeal vascularization. Together, these data identify an unexpected lymphatic lineage and developmental mechanism necessary for establishing normal meningeal blood vasculature.
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Base de dados:
MEDLINE
Assunto principal:
Peixe-Zebra
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Neovascularização Fisiológica
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Proteínas de Peixe-Zebra
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Células Endoteliais
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Fatores de Crescimento do Endotélio Vascular
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Meninges
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article