Assaying Spontaneous Network Activity and Cellular Viability Using Multi-well Microelectrode Arrays.
Methods Mol Biol
; 1601: 153-170, 2017.
Article
em En
| MEDLINE
| ID: mdl-28470525
ABSTRACT
Microelectrode array (MEA) technology is a neurophysiological method that allows for the spontaneous measure of activity in neural cultures and determination of drug and chemical effects thereon. Recent introduction of multi-well MEA (mwMEA) formats have dramatically increased the throughput of this technology, allowing more efficient compound screening. Rapid characterization of compounds for neuroactivity or neurotoxicity hazard evaluation following acute, chronic, or developmental exposures ideally would also consider compound effects on cell health, and to do so in the same well requires a multiplexed approach. Procedures describing the multiplexed method to acute and developmental screening are described in this chapter.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Sobrevivência Celular
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Testes de Toxicidade
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Citotoxinas
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Análise em Microsséries
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Microeletrodos
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Rede Nervosa
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Neurônios
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article