A bioluminescent imaging mouse model for Marburg virus based on a pseudovirus system.
Hum Vaccin Immunother
; 13(8): 1811-1817, 2017 08 03.
Article
em En
| MEDLINE
| ID: mdl-28481728
ABSTRACT
Marburg virus (MARV) can cause lethal hemorrhagic fever in humans. Handling of MARV is restricted to high-containment biosafety level 4 (BSL-4) facilities, which greatly impedes research into this virus. In this study, a high titer of MARV pseudovirus was generated through optimization of the HIV backbone vectors, the ratio of backbone vector to MARV glycoprotein expression vector, and the transfection reagents. An in vitro neutralization assay and an in vivo bioluminescent imaging mouse model for MARV were developed based on the pseudovirus. Protective serum against MARV was successfully induced in guinea pigs, which showed high neutralization activity in vitro and could also protect Balb/c mice from MARV pseudovirus infection in vivo. This system could be a convenient tool to enable the evaluation of vaccines and therapeutic drugs against MARV in non-BSL-4 laboratories.
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Base de dados:
MEDLINE
Assunto principal:
Vacinas Virais
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Medições Luminescentes
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Marburgvirus
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Doença do Vírus de Marburg
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article