Evidence for the subsynaptic zone as a preferential site for CHRN recycling at neuromuscular junctions.
Small GTPases
; 10(5): 395-402, 2019 09.
Article
em En
| MEDLINE
| ID: mdl-28489965
ABSTRACT
Vertebrate skeletal muscle contraction is mediated by nicotinic acetylcholine receptors (CHRN). Endocytosis and recycling of CHRN regulate their proper abundance at nerve-muscle synapses, i.e. neuromuscular junctions. Recent work showed that RAB5 is essential for CHRN endocytosis. Here, using in vivo-imaging of endocytosed CHRN and RAB-GFP fusion proteins, we deliver evidence for differential effects of RAB5-GFP, RAB4-GFP, and RAB11-GFP on CHRN endocytosis. Furthermore, while newly endocytosed CHRN colocalized with RAB5-GFP over large stretches of muscle fibers, RAB4-GFP and RAB11-GFP colocalized with endocytosed CHRN almost exclusively at neuromuscular junctions. In agreement with previous findings, this data suggests the existence of a specialized subsynaptic zone that is particularly relevant for CHRN recycling.
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MEDLINE
Assunto principal:
Receptores Nicotínicos
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Músculo Esquelético
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Proteínas rab de Ligação ao GTP
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Endocitose
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Junção Neuromuscular
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article