STAT4 expression and activation is increased during mitosis in vitro and in vivo in skin- and mucosa-derived cell types: implications in neoplastic and inflammatory skin diseases.
J Eur Acad Dermatol Venereol
; 31(10): 1663-1673, 2017 Oct.
Article
em En
| MEDLINE
| ID: mdl-28516569
ABSTRACT
BACKGROUND:
The signal transducer and activator of transcription-4 (STAT4/Stat4) is a transcription factor known to convey signals from interleukin-12, interleukin-23, and interferon-alpha/beta to the nucleus, resulting in activation of dendritic cells, T-helper cell differentiation and production of interferon-gamma.OBJECTIVE:
To demonstrate a novel role for STAT4 in cell mitosis.RESULTS:
Phosphoserine STAT4 (pSerSTAT4) is increased in cells undergoing mitosis and is distributed throughout the cytoplasm during this stage of the cell cycle, whilst phosphotyrosine STAT4 (pTyrSTAT4) is confined to the chromosomal compartment. This distinct pattern of pSerSTAT4 during mitosis is seen in vitro in human keratinocytes and in other cell types. This is also present in vivo in cells undergoing mitosis in normal skin, psoriasis and squamous cell carcinoma. Inhibition of STAT4 phosphorylation by lisofylline and depletion of STAT4 by RNA interference results in a delay in progression of mitosis and leads to a reduction in cells completing cytokinesis.CONCLUSION:
Our data demonstrate that STAT4 plays a role in enabling the normal and timely division of cells undergoing mitosis.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Pele
/
Neoplasias Cutâneas
/
Dermatite
/
Fator de Transcrição STAT4
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Mitose
/
Mucosa
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article