Blocking promiscuous activation at cryptic promoters directs cell type-specific gene expression.
Science
; 356(6339): 717-721, 2017 05 19.
Article
em En
| MEDLINE
| ID: mdl-28522526
ABSTRACT
To selectively express cell type-specific transcripts during development, it is critical to maintain genes required for other lineages in a silent state. Here, we show in the Drosophila male germline stem cell lineage that a spermatocyte-specific zinc finger protein, Kumgang (Kmg), working with the chromatin remodeler dMi-2 prevents transcription of genes normally expressed only in somatic lineages. By blocking transcription from normally cryptic promoters, Kmg restricts activation by Aly, a component of the testis-meiotic arrest complex, to transcripts for male germ cell differentiation. Our results suggest that as new regions of the genome become open for transcription during terminal differentiation, blocking the action of a promiscuous activator on cryptic promoters is a critical mechanism for specifying precise gene activation.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Especificidade de Órgãos
/
Regulação da Expressão Gênica
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Regiões Promotoras Genéticas
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Drosophila melanogaster
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article