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Directed Differentiation of Human Pluripotent Stem Cells to Microglia.
Douvaras, Panagiotis; Sun, Bruce; Wang, Minghui; Kruglikov, Ilya; Lallos, Gregory; Zimmer, Matthew; Terrenoire, Cecile; Zhang, Bin; Gandy, Sam; Schadt, Eric; Freytes, Donald O; Noggle, Scott; Fossati, Valentina.
Afiliação
  • Douvaras P; The New York Stem Cell Foundation Research Institute, New York, NY 10019, USA. Electronic address: pdouvaras@nyscf.org.
  • Sun B; The New York Stem Cell Foundation Research Institute, New York, NY 10019, USA.
  • Wang M; Department of Genetics and Genomic Sciences, Icahn Institute for Genomics and Multiscale Biology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
  • Kruglikov I; The New York Stem Cell Foundation Research Institute, New York, NY 10019, USA.
  • Lallos G; The New York Stem Cell Foundation Research Institute, New York, NY 10019, USA.
  • Zimmer M; The New York Stem Cell Foundation Research Institute, New York, NY 10019, USA.
  • Terrenoire C; The New York Stem Cell Foundation Research Institute, New York, NY 10019, USA.
  • Zhang B; Department of Genetics and Genomic Sciences, Icahn Institute for Genomics and Multiscale Biology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
  • Gandy S; Department of Neurology, Department of Psychiatry, Alzheimer's Disease Research Center, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
  • Schadt E; Department of Genetics and Genomic Sciences, Icahn Institute for Genomics and Multiscale Biology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
  • Freytes DO; The Joint Department of Biomedical Engineering, North Carolina State University and University of North Carolina-Chapel Hill, Raleigh, NC 27695, USA.
  • Noggle S; The New York Stem Cell Foundation Research Institute, New York, NY 10019, USA.
  • Fossati V; The New York Stem Cell Foundation Research Institute, New York, NY 10019, USA.
Stem Cell Reports ; 8(6): 1516-1524, 2017 06 06.
Article em En | MEDLINE | ID: mdl-28528700
ABSTRACT
Microglia, the immune cells of the brain, are crucial to proper development and maintenance of the CNS, and their involvement in numerous neurological disorders is increasingly being recognized. To improve our understanding of human microglial biology, we devised a chemically defined protocol to generate human microglia from pluripotent stem cells. Myeloid progenitors expressing CD14/CX3CR1 were generated within 30 days of differentiation from both embryonic and induced pluripotent stem cells (iPSCs). Further differentiation of the progenitors resulted in ramified microglia with highly motile processes, expressing typical microglial markers. Analyses of gene expression and cytokine release showed close similarities between iPSC-derived (iPSC-MG) and human primary microglia as well as clear distinctions from macrophages. iPSC-MG were able to phagocytose and responded to ADP by producing intracellular Ca2+ transients, whereas macrophages lacked such response. The differentiation protocol was highly reproducible across several pluripotent stem cell lines.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Microglia / Células-Tronco Pluripotentes Idioma: En Ano de publicação: 2017 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Microglia / Células-Tronco Pluripotentes Idioma: En Ano de publicação: 2017 Tipo de documento: Article