Anti-inflammatory activities of compounds from twigs of Morus alba.
Fitoterapia
; 120: 17-24, 2017 Jul.
Article
em En
| MEDLINE
| ID: mdl-28529178
ABSTRACT
Five new compounds, 10-oxomornigrol F (1), (7â³R)-(-)-6-(7â³-hydroxy-3â³,8â³-dimethyl-2â³,8â³-octadien-1â³-yl)apigenin (2), ramumorin A (3), ramumorin B (4), and (4S,7S,8R)-trihydroxyoctadeca-5Z-enoic acid (5), together with 31 known compounds (6-36), were isolated from the twigs of Morus alba (Moraceae). The chemical structures of these compounds were established using spectroscopic analyses, 1D and 2D NMR, high-resolution electrospray ionization mass spectrometry (HRESIMS), and Mosher's methods. The anti-inflammatory activities of the compounds were evaluated by investigating their ability to inhibit lipopolysaccharide (LPS)-induced nitric oxide (NO) production in macrophage RAW 264.7 cells. Compounds 1, 2, 13, 17, 19, 25-28, and 32 showed inhibitory effects with IC50 values ranging from 2.2 to 5.3µg/mL. Compounds 1, 2, 17, 25, and 32 reduced LPS-induced inducible nitric oxide synthase (iNOS) expression in a concentration-dependent manner. In addition, pretreating the cells with compound 1, 17, and 32 significantly suppressed LPS-induced expression of cyclooxygenase-2 (COX-2) protein.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Flavonoides
/
Morus
/
Anti-Inflamatórios
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article