Blue fluorescent amino acid for biological spectroscopy and microscopy.
Proc Natl Acad Sci U S A
; 114(23): 6005-6009, 2017 06 06.
Article
em En
| MEDLINE
| ID: mdl-28533371
ABSTRACT
Many fluorescent proteins are currently available for biological spectroscopy and imaging measurements, allowing a wide range of biochemical and biophysical processes and interactions to be studied at various length scales. However, in applications where a small fluorescence reporter is required or desirable, the choice of fluorophores is rather limited. As such, continued effort has been devoted to the development of amino acid-based fluorophores that do not require a specific environment and additional time to mature and have a large fluorescence quantum yield, long fluorescence lifetime, good photostability, and an emission spectrum in the visible region. Herein, we show that a tryptophan analog, 4-cyanotryptophan, which differs from tryptophan by only two atoms, is the smallest fluorescent amino acid that meets these requirements and has great potential to enable in vitro and in vivo spectroscopic and microscopic measurements of proteins.
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Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Fluorescência
/
Análise Espectral
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Corantes Fluorescentes
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article