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Bone Regeneration Induced by Bone Porcine Block with Bone Marrow Stromal Stem Cells in a Minipig Model of Mandibular "Critical Size" Defect.
Scarano, Antonio; Crincoli, Vito; Di Benedetto, Adriana; Cozzolino, Valerio; Lorusso, Felice; Podaliri Vulpiani, Michele; Grano, Maria; Kalemaj, Zamira; Mori, Giorgio; Grassi, Felice Roberto.
Afiliação
  • Scarano A; Department of Medical, Oral and Biotechnological Sciences and CeSI-Met, University of Chieti-Pescara, Chieti, Italy.
  • Crincoli V; Interdisciplinary Department of Medicine, University of Bari "A. Moro", Bari, Italy.
  • Di Benedetto A; Department of Clinical and Experimental Medicine, Medical School, University of Foggia, Foggia, Italy.
  • Cozzolino V; Department of Medical, Oral and Biotechnological Sciences and CeSI-Met, University of Chieti-Pescara, Chieti, Italy.
  • Lorusso F; Department of Medical, Oral and Biotechnological Sciences and CeSI-Met, University of Chieti-Pescara, Chieti, Italy.
  • Podaliri Vulpiani M; Department of Animals Sperimentation, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale", Teramo, Italy.
  • Grano M; Department of Emergency and Organ Transplantation, University of Bari "A. Moro", Bari, Italy.
  • Kalemaj Z; Department of Surgical Sciences, Dental School, University of Turin, Torino, Italy.
  • Mori G; Department of Clinical and Experimental Medicine, Medical School, University of Foggia, Foggia, Italy.
  • Grassi FR; Department of Basic and Medical Sciences, Neurosciences and Sense Organs, University of Bari, Bari, Italy.
Stem Cells Int ; 2017: 9082869, 2017.
Article em En | MEDLINE | ID: mdl-28553359
Introduction. Adding stem cells to biodegradable scaffolds to enhance bone regeneration is a valuable option. Different kinds of stem cells with osteoblastic activity were tested, such as bone marrow stromal stem cells (BMSSCs). Aim. To assess a correct protocol for osteogenic stem cell differentiation, so BMSSCs were seeded on a bone porcine block (BPB). Materials and Methods. Bone marrow from six minipigs was extracted from tibiae and humeri and treated to isolate BMSSCs. After seeding on BPB, critical-size defects were created on each mandible of the minipigs and implanted with BPB and BPB/BMSSCs. After three months, histomorphometric analysis was performed. Results. Histomorphometric analysis provided percentages of the three groups. Tissues present in control defects were 23 ± 2% lamellar bone, 28 ± 1% woven bone, and 56 ± 4% marrow spaces; in BPB defects were 20 ± 5% BPB, 32 ± 2% lamellar bone, 24 ± 1% woven bone, and 28 ± 2% marrow spaces; in BPB/BMSSCs defects were 17 ± 4% BPB/BMSSCs, 42 ± 2% lamellar bone, 12 ± 1% woven bone, and 22 ± 3% marrow spaces. Conclusion. BPB used as a scaffold to induce bone regeneration may benefit from the addition of BDPSCs in the tissue-engineered constructs.

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2017 Tipo de documento: Article