Comparative study of three phenoloxidases in the sea cucumber Apostichopus japonicus.
Fish Shellfish Immunol
; 67: 11-18, 2017 Aug.
Article
em En
| MEDLINE
| ID: mdl-28554836
ABSTRACT
In order to preliminarily illustrate the functional differences of phenoloxidases (POs) in Apostichopus japonicus, the full-length cDNAs of two POs (named as AjPOâ
¡ and AjPOâ
¢, respectively) were cloned from the coelomocytes of A. japonicus using 3'- and 5'-rapid amplification of cDNA ends method, and combined with the previously acquired full-length cDNA of a laccase-type PO from A. japonicus (Accession No. KF040052, named as AjPOâ
), the sequence structure and phylogenic status of POs from A. japonicus (AjPOs) were comparatively analyzed, and the transcriptional expression of AjPOs in different tissues, at different developmental stages and after different bacterial challenges was determined with quantitative real-time PCR method. Sequence analysis indicated AjPOâ
¡ and AjPOâ
¢ were both laccase-type POs, coincident to the results of phylogenic analysis. Sequence analysis also showed that AjPOâ
had a transmembrane domain (J. Jiang et al., 2014), AjPOâ
¡ contained a signal peptide, and AjPOâ
¢ possessed a signal peptide and a transmembrane domain, implying that three AjPOs might play different roles in immune and physiological processes. Transcriptional expression analysis showed that AjPOâ
¡ and AjPOâ
¢ were most abundant in tube feet, while AjPOâ
had the highest expression level in coelomocytes (J. Jiang et al., 2014), suggesting that AjPOâ
may be mainly involved in immune response, while AjPOâ
¡ and AjPOâ
¢ are probably responsible for other physiological processes in addition to immune response. Besides, three AjPOs were determined to have different expression patterns during organism development and different spectrums of response against bacteria, which further indicated that there might be immune and physiological functional differentiation among three AjPOs.
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Base de dados:
MEDLINE
Assunto principal:
Expressão Gênica
/
Monofenol Mono-Oxigenase
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Stichopus
/
Imunidade Inata
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article