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Detection of Gene Rearrangements in Circulating Tumor Cells: Examples of ALK-, ROS1-, RET-Rearrangements in Non-Small-Cell Lung Cancer and ERG-Rearrangements in Prostate Cancer.
Catelain, Cyril; Pailler, Emma; Oulhen, Marianne; Faugeroux, Vincent; Pommier, Anne-Laure; Farace, Françoise.
Afiliação
  • Catelain C; Gustave Roussy, Université Paris-Saclay, "Circulating Tumor Cells" Translational Platform, AMMICA CNRS UMS3655 - INSERM US23, 114 rue Édouard Vaillant, F-94805, Villejuif, France.
  • Pailler E; INSERM, U981 "Identification of Molecular Predictors and New Targets for Cancer Treatment", 114 rue Édouard Vaillant, F-94805, Villejuif, France.
  • Oulhen M; Gustave Roussy, Université Paris-Saclay, "Circulating Tumor Cells" Translational Platform, AMMICA CNRS UMS3655 - INSERM US23, 114 rue Édouard Vaillant, F-94805, Villejuif, France.
  • Faugeroux V; INSERM, U981 "Identification of Molecular Predictors and New Targets for Cancer Treatment", 114 rue Édouard Vaillant, F-94805, Villejuif, France.
  • Pommier AL; Univ Paris Sud, Université Paris-Saclay, 114 rue Édouard Vaillant, F-94270, Le Kremlin-Bicetre, France.
  • Farace F; Gustave Roussy, Université Paris-Saclay, "Circulating Tumor Cells" Translational Platform, AMMICA CNRS UMS3655 - INSERM US23, 114 rue Édouard Vaillant, F-94805, Villejuif, France.
Adv Exp Med Biol ; 994: 169-179, 2017.
Article em En | MEDLINE | ID: mdl-28560674
ABSTRACT
Circulating tumor cells (CTCs) hold promise as biomarkers to aid in patient treatment stratification and disease monitoring. Because the number of cells is a critical parameter for exploiting CTCs for predictive biomarker's detection, we developed a FISH (fluorescent in situ hybridization) method for CTCs enriched on filters (filter-adapted FISH [FA-FISH]) that was optimized for high cell recovery. To increase the feasibility and reliability of the analyses, we combined fluorescent staining and FA-FISH and developed a semi-automated microscopy method for optimal FISH signal identification in filtration-enriched CTCs . Here we present these methods and their use for the detection and characterization of ALK-, ROS1-, RET-rearrangement in CTCs from non-small-cell lung cancer and ERG-rearrangements in CTCs from prostate cancer patients.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias da Próstata / Proteínas Tirosina Quinases / Rearranjo Gênico / Proteínas Proto-Oncogênicas / Receptores Proteína Tirosina Quinases / Carcinoma Pulmonar de Células não Pequenas / Proteínas Proto-Oncogênicas c-ret / Neoplasias Pulmonares / Células Neoplásicas Circulantes Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias da Próstata / Proteínas Tirosina Quinases / Rearranjo Gênico / Proteínas Proto-Oncogênicas / Receptores Proteína Tirosina Quinases / Carcinoma Pulmonar de Células não Pequenas / Proteínas Proto-Oncogênicas c-ret / Neoplasias Pulmonares / Células Neoplásicas Circulantes Idioma: En Ano de publicação: 2017 Tipo de documento: Article