Impact of oocyte-secreted factors on its developmental competence in buffalo.
Zygote
; 25(3): 313-320, 2017 Jun.
Article
em En
| MEDLINE
| ID: mdl-28592334
ABSTRACT
Oocyte-secreted factors (OSFs) play an important role in the acquisition of oocyte developmental competence through bidirectional cross-talk between oocyte and cumulus cells via gap junctions. Thus, the present study was designed to investigate the effect of two OSFs, growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15), on the developmental competence of buffalo oocytes derived from two different follicle sizes. Cumulus-oocyte complexes (COCs) from large follicles (LF, >6 mm) or small follicles (SF, 0.05) between DOs and combination groups. Relative mRNA analysis revealed significantly higher (P > 0.05) expression of the cumulus cell marker genes EGFR, HAS2, and CD44 in LF-derived than SF-derived oocyte; the expression of these markers was significantly higher (P > 0.05) in DOs and combination groups, irrespective of the follicle size. These results suggested that LF-derived oocytes have a higher developmental competence than SF-derived oocytes and that supplementation of GDF9 and BMP15 modulates the developmental competence of buffalo oocytes by increasing the relative abundance of cumulus-enabling factors and thereby increasing cleavage and the quality of blastocyst production.
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Base de dados:
MEDLINE
Assunto principal:
Oócitos
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Regulação da Expressão Gênica
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Proteína Morfogenética Óssea 15
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Fator 9 de Diferenciação de Crescimento
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article