Detection and Typing of "Candidatus Phytoplasma " spp. in Host DNA Extracts Using Oligonucleotide-Coupled Fluorescent Microspheres.
Methods Mol Biol
; 1616: 121-136, 2017.
Article
em En
| MEDLINE
| ID: mdl-28600765
ABSTRACT
The use of oligonucleotide-coupled fluorescent microspheres is a rapid, sequencing-independent, and reliable way to diagnose bacterial diseases. Previously described applications of oligonucleotide-coupled fluorescent microspheres for the detection and identification of bacteria in human clinical samples have been successfully adapted to detect and differentiate "Ca. Phytoplasma" species using as a target the chaperonin 60-encoding gene. In this chapter, we describe in detail the design and validation of oligonucleotide capture probes, and their application in the assay aiming to differentiate phytoplasma strains infecting Brassica napus and Camelina sativa plants grown in the same geographic location at the same time.
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MEDLINE
Assunto principal:
Doenças das Plantas
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Sondas de Oligonucleotídeos
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Hibridização In Situ
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Phytoplasma
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article