Application of C8 liquid chromatography-tandem mass spectrometry for the analysis of enniatins and bassianolides.
J Chromatogr A
; 1508: 65-72, 2017 Jul 28.
Article
em En
| MEDLINE
| ID: mdl-28619584
ABSTRACT
Several moderately pathogenic Fusarium species produce enniatins, a family of cyclohexadepsipeptides with insecticidal and phytotoxic activities. A semi-targeted LC-MS/MS approach utilizing reversed-phase C8 UHPLC column chromatography that combines both product ion and neutral loss filtering was developed to detect enniatins and structurally-related compounds in a sample. Using this methodology, resolution of all major enniatins was achieved with a 6-min LC run. From extracts of F. avenaceum, F. acuminatum and F. subglutinans, 23 enniatins and 16 previously unreported bassianolide-like cyclooctadepsipeptides were detected. Enniatins B, B1, A1 and A were the most frequently detected enniatins from silage and inoculated maize. The newly discovered bassianolide compounds were also detected, in lesser amounts on inoculated maize and in naturally contaminated silage.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Peptídeos Cíclicos
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Cromatografia Líquida
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Depsipeptídeos
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Espectrometria de Massas em Tandem
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Fusarium
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article