The suitability of Tórtora's medium for the production of enterotoxin in Clostridium perfringens strains.
Microbiol Immunol
; 29(6): 509-16, 1985.
Article
em En
| MEDLINE
| ID: mdl-2864626
ABSTRACT
Examination of 200 samples from soil and the same number of samples from healthy human feces yielded 49 (24.5%) and 105 (52.5%) strains of heat-resistant Clostridium perfringens respectively. Fourteen (7.0%) strains isolated from soil and 37 (18.5%) from feces synthesized enterotoxin, as demonstrated by Tórtora's method, at sufficient levels to permit its detection by mouse lethality, microslide double gel diffusion or counterimmunoelectrophoresis tests. By using the Duncan-Strong (DS) method, only four (2%) enterotoxigenic strains from soil and 14 (7.0%) from feces were obtained. The supernatant fluid from two enterotoxigenic-negative strains grown in DS medium gave a false-positive reaction when they were injected intravenously into mice. Tórtora's medium was preferable because a larger number of isolated strains produced spores and enterotoxin to permit their recognition as enterotoxigenic strains.
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Base de dados:
MEDLINE
Assunto principal:
Clostridium perfringens
/
Meios de Cultura
/
Enterotoxinas
Idioma:
En
Ano de publicação:
1985
Tipo de documento:
Article