Your browser doesn't support javascript.
loading
Enrichment and fluorogenic labelling of 5-formyluracil in DNA.
Liu, Chaoxing; Wang, Yafen; Zhang, Xiong; Wu, Fan; Yang, Wei; Zou, Guangrong; Yao, Qian; Wang, Jiaqi; Chen, Yuqi; Wang, Shaoru; Zhou, Xiang.
Afiliação
  • Liu C; College of Chemistry and Molecular Sciences , Key Laboratory of Biomedical Polymers of Ministry of Education , The Institute for Advanced Studies , Wuhan University , Hubei Province Key Laboratory of Allergy and Immunology , Wuhan , Hubei 430072 , P. R. China . Email: xzhou@whu.edu.cn ; ; Tel: +86-2
  • Wang Y; College of Chemistry and Molecular Sciences , Key Laboratory of Biomedical Polymers of Ministry of Education , The Institute for Advanced Studies , Wuhan University , Hubei Province Key Laboratory of Allergy and Immunology , Wuhan , Hubei 430072 , P. R. China . Email: xzhou@whu.edu.cn ; ; Tel: +86-2
  • Zhang X; College of Chemistry and Molecular Sciences , Key Laboratory of Biomedical Polymers of Ministry of Education , The Institute for Advanced Studies , Wuhan University , Hubei Province Key Laboratory of Allergy and Immunology , Wuhan , Hubei 430072 , P. R. China . Email: xzhou@whu.edu.cn ; ; Tel: +86-2
  • Wu F; College of Chemistry and Molecular Sciences , Key Laboratory of Biomedical Polymers of Ministry of Education , The Institute for Advanced Studies , Wuhan University , Hubei Province Key Laboratory of Allergy and Immunology , Wuhan , Hubei 430072 , P. R. China . Email: xzhou@whu.edu.cn ; ; Tel: +86-2
  • Yang W; College of Chemistry and Molecular Sciences , Key Laboratory of Biomedical Polymers of Ministry of Education , The Institute for Advanced Studies , Wuhan University , Hubei Province Key Laboratory of Allergy and Immunology , Wuhan , Hubei 430072 , P. R. China . Email: xzhou@whu.edu.cn ; ; Tel: +86-2
  • Zou G; College of Chemistry and Molecular Sciences , Key Laboratory of Biomedical Polymers of Ministry of Education , The Institute for Advanced Studies , Wuhan University , Hubei Province Key Laboratory of Allergy and Immunology , Wuhan , Hubei 430072 , P. R. China . Email: xzhou@whu.edu.cn ; ; Tel: +86-2
  • Yao Q; College of Chemistry and Molecular Sciences , Key Laboratory of Biomedical Polymers of Ministry of Education , The Institute for Advanced Studies , Wuhan University , Hubei Province Key Laboratory of Allergy and Immunology , Wuhan , Hubei 430072 , P. R. China . Email: xzhou@whu.edu.cn ; ; Tel: +86-2
  • Wang J; College of Chemistry and Molecular Sciences , Key Laboratory of Biomedical Polymers of Ministry of Education , The Institute for Advanced Studies , Wuhan University , Hubei Province Key Laboratory of Allergy and Immunology , Wuhan , Hubei 430072 , P. R. China . Email: xzhou@whu.edu.cn ; ; Tel: +86-2
  • Chen Y; College of Chemistry and Molecular Sciences , Key Laboratory of Biomedical Polymers of Ministry of Education , The Institute for Advanced Studies , Wuhan University , Hubei Province Key Laboratory of Allergy and Immunology , Wuhan , Hubei 430072 , P. R. China . Email: xzhou@whu.edu.cn ; ; Tel: +86-2
  • Wang S; College of Chemistry and Molecular Sciences , Key Laboratory of Biomedical Polymers of Ministry of Education , The Institute for Advanced Studies , Wuhan University , Hubei Province Key Laboratory of Allergy and Immunology , Wuhan , Hubei 430072 , P. R. China . Email: xzhou@whu.edu.cn ; ; Tel: +86-2
  • Zhou X; College of Chemistry and Molecular Sciences , Key Laboratory of Biomedical Polymers of Ministry of Education , The Institute for Advanced Studies , Wuhan University , Hubei Province Key Laboratory of Allergy and Immunology , Wuhan , Hubei 430072 , P. R. China . Email: xzhou@whu.edu.cn ; ; Tel: +86-2
Chem Sci ; 8(6): 4505-4510, 2017 Jun 01.
Article em En | MEDLINE | ID: mdl-28660064
ABSTRACT
Recently, the detection of natural thymine modified 5-formyluracil has attracted widespread attention. Herein, we introduce a new insight into designing reagents for both the selective biotin enrichment and fluorogenic labelling of 5-formyluracil in DNA. Biotinylated o-phenylenediamine directly tethered to naphthalimide can switch on 5-formyluracil, under physiological conditions, which can then be used in cell imaging after exposure to γ-irradiation. In addition, its labelling property caused the polymerase extension to stop in the 5-formyluracil site, which gave us more information than the fluorescence did itself. The idea of detecting 5-formyluracil might be used in the synthesis of other modified diaminofluoresceins.
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2017 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2017 Tipo de documento: Article