miR199a3p is involved in the pathogenesis and progression of diabetic neuropathy through downregulation of SerpinE2.
Mol Med Rep
; 16(3): 2417-2424, 2017 09.
Article
em En
| MEDLINE
| ID: mdl-28677735
ABSTRACT
The present study aimed to investigate the expression status of miRNA199a3p in patients with diabetic neuropathy (DN) and the mechanism by which this miRNA is involved in the genesis of DN. The expression of miRNA199a3p in plasma of peripheral blood was compared between patients with diabetes and a family history of diabetes and control volunteers by reverse transcriptionquantitative polymerase chain reaction (RTqPCR); in 60 diabetes patients, 45 (75%) demosntrated upregulated miR199a3p expression compared with control volunteer plasma. RTqPCR was also used to detect miRNA199a3p expression in paired lower limb skin tissues from 30 patients with DN and 20 control volunteers; miR199a3p expression in patients with DN was significantly higher than in the control group. Next miR199a3p expression levels were evaluated with respect to the clinicpathological parameters of diabetes; increased expression of miR199a3p was significantly associated with increased disease duration (P=0.041), glycated hemoglobin (HbA1C) levels (P=0.033), and fibrinogen levels (P=0.003). Finally, the effects on downstream mRNA expression levels were investigated as a result of manipulating miR199a3p levels. miR199a3p overexpression inhibited the expression of the extracellular serine protease inhibitor E2 (SerpinE2). Therefore, it may be hypothesized that miR199a3p can induce DN via promoting coagulation in skin peripheral circulation, through the downregulation of SerpinE2. The present findings suggested that miR199a3p may have potential as a novel therapeutic target for the treatment of patients with DN.
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Base de dados:
MEDLINE
Assunto principal:
MicroRNAs
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Diabetes Mellitus Tipo 2
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Neuropatias Diabéticas
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Serpina E2
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article