Protective role of vitamin E preconditioning of human dermal fibroblasts against thermal stress in vitro.
Life Sci
; 184: 1-9, 2017 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-28684064
ABSTRACT
AIMS:
Oxidative microenvironment of burnt skin restricts the outcome of cell based therapies of thermal skin injuries. The aim of this study was to precondition human dermal fibroblasts with an antioxidant such as vitamin E to improve their survival and therapeutic abilities in heat induced oxidative in vitro environment. MAINMETHODS:
Fibroblasts were treated with 100µM vitamin E for 24h at 37°C followed by heat shock for 10min at 51°C in fresh serum free medium. KEYFINDINGS:
Preconditioning with vitamin E reduced cell injury as demonstrated by decreased expression of annexin-V, cytochrome p450 (CYP450) mediated oxidative reactions, senescence and release of lactate dehydrogenase (LDH) accomplished by down-regulated expression of pro-apoptotic BAX gene. Vitamin E preconditioned cells exhibited remarkable improvement in cell viability, release of paracrine factors such as epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), stromal derived factor-1alpha (SDF-1α) and also showed significantly up-regulated levels of PCNA, VEGF, BCL-XL, FGF7, FGF23, FLNß and Col7α genes presumably through activation of phosphatidylinositol 3-kinase (PI3-K)/Akt pathway.SIGNIFICANCE:
The results suggest that pretreatment of fibroblasts with vitamin E prior to transplantation in burnt skin speeds up the wound healing process by improving the antioxidant scavenging responses in oxidative environment of transplanted burn wounds.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Estresse Fisiológico
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Vitamina E
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Cicatrização
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Fibroblastos
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Antioxidantes
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article