Ribbon scanning confocal for high-speed high-resolution volume imaging of brain.
PLoS One
; 12(7): e0180486, 2017.
Article
em En
| MEDLINE
| ID: mdl-28686653
Whole-brain imaging is becoming a fundamental means of experimental insight; however, achieving subcellular resolution imagery in a reasonable time window has not been possible. We describe the first application of multicolor ribbon scanning confocal methods to collect high-resolution volume images of chemically cleared brains. We demonstrate that ribbon scanning collects images over ten times faster than conventional high speed confocal systems but with equivalent spectral and spatial resolution. Further, using this technology, we reconstruct large volumes of mouse brain infected with encephalitic alphaviruses and demonstrate that regions of the brain with abundant viral replication were inaccessible to vascular perfusion. This reveals that the destruction or collapse of large regions of brain micro vasculature may contribute to the severe disease caused by Venezuelan equine encephalitis virus. Visualization of this fundamental impact of infection would not be possible without sampling at subcellular resolution within large brain volumes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Encéfalo
/
Microscopia Confocal
/
Vírus da Encefalite Equina Venezuelana
/
Encefalomielite Equina Venezuelana
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article