Adipose stem cells enhance myoblast proliferation via acetylcholine and extracellular signal-regulated kinase 1/2 signaling.
Muscle Nerve
; 57(2): 305-311, 2018 02.
Article
em En
| MEDLINE
| ID: mdl-28686790
ABSTRACT
INTRODUCTION:
In this study we investigated the interaction between adipose tissue-derived stem cells (ASCs) and myoblasts in co-culture experiments.METHODS:
Specific inductive media were used to differentiate ASCs in vitro into a Schwann cell-like phenotype (differentiated adipose tissue-derived stem cells, or dASCs) and, subsequently, the expression of acetylcholine (ACh)-related machinery was determined. In addition, the expression of muscarinic ACh receptors was examined in denervated rat gastrocnemius muscles.RESULTS:
In contrast to undifferentiated ASCs, dASCs expressed more choline acetyltransferase and vesicular acetylcholine transporter. When co-cultured with myoblasts, dASCs enhanced the proliferation rate, as did ACh administration alone. Western blotting and pharmacological inhibitor studies showed that phosphorylated extracellular signal-regulated kinase 1/2 signaling mediated these effects. In addition, denervated muscle showed higher expression of muscarinic ACh receptors than control muscle.DISCUSSION:
Our findings suggest that dASCs promote proliferation of myoblasts through paracrine secretion of ACh, which could explain some of their regenerative capacity in vivo. Muscle Nerve 57 305-311, 2018.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Acetilcolina
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Adipócitos
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Sistema de Sinalização das MAP Quinases
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Mioblastos
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Transplante de Células-Tronco
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article