Rapid Identification Method of Omphalotus japonicus by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP).
Shokuhin Eiseigaku Zasshi
; 58(3): 113-123, 2017.
Article
em Ja
| MEDLINE
| ID: mdl-28690301
ABSTRACT
Omphalotus japonicus is a poisonous mushroom that grows in Japan. It can be mistaken for edible mushrooms (Shiitake, Hiratake and Mukitake), and if ingested, it causes food poisoning within 30 min to 1 hr. We established a rapid detection method using PCR-RFLP to identify O. japonicus by restriction digestion of the amplified ITS region. By using Sau96I, Bpu10I, SfcI or DrdI/HincII as a restriction enzyme, it was possible to rapidly identify and discriminate O. japonicus based on the fragment length. This study also provided a short PCR-RFLP system comprising amplification and digestion of a short 200-bp DNA fragment within the ITS region. The system could identify and discriminate O. japonicus after in vitro gastric digestion of native and heated mushroom samples as a model of food poisoning. In addition, a confirmatory assay using real-time PCR was developed to achieve more sensitive detection of O. japonicus.
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Base de dados:
MEDLINE
Assunto principal:
Polimorfismo de Fragmento de Restrição
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Agaricales
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Reação em Cadeia da Polimerase em Tempo Real
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Análise de Alimentos
Idioma:
Ja
Ano de publicação:
2017
Tipo de documento:
Article