PARP-1 Interaction with and Activation by Histones and Nucleosomes.
Methods Mol Biol
; 1608: 255-267, 2017.
Article
em En
| MEDLINE
| ID: mdl-28695515
ABSTRACT
Poly(ADP-ribose) Polymerase 1 (PARP-1) is an abundant chromatin associated protein, typical for most eukaryotic nuclei. The localization of PARP-1 in chromatin and its enzymatic activation involves multiple interactions of PARP-1 with nucleosomal histones, other proteins, and DNA. We report a set of methods designed to reconstitute PARP-1 regulation in vitro. These methods involve the expression of PARP-1 and PARP-1-regulating proteins using bacterial and eukaryotic systems, purification of these proteins using chromatography, testing of individual interactions in vitro, assembly of active complexes, and reconstitution of PARP-1 regulating reactions in vitro.
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Base de dados:
MEDLINE
Assunto principal:
Histonas
/
Nucleossomos
/
Poli(ADP-Ribose) Polimerase-1
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article