Prevalence of cervical colonization by Ureaplasma parvum, Ureaplasma urealyticum, Mycoplasma hominis and Mycoplasma genitalium in childbearing age women by a commercially available multiplex real-time PCR: An Italian observational multicentre study.
J Microbiol Immunol Infect
; 51(2): 220-225, 2018 Apr.
Article
em En
| MEDLINE
| ID: mdl-28711440
ABSTRACT
BACKGROUND:
Mycoplasmas are frequently isolated from the genital tract. New molecular PCR-based methods for the detection of mycoplasmas can better define the real epidemiology of these microorganisms. The aim of this study was to evaluate the prevalence of mycoplasmas in a population of childbearing age women by means of PCR.METHODS:
This 21-month multicentre observational study was conducted at four Italian clinical microbiology laboratories. Women reporting symptoms of vaginitis/cervicitis, or with history of infertility, pregnancy, miscarriage or preterm birth were included. Detection of Ureaplasma parvum, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium was performed from cervical swabs by means of a commercially available multiplex real-time PCR.RESULTS:
a total of 1761 women fulfilled the inclusion criteria and were included in the study. The overall prevalence was U. parvum 38.3%, U. urealyticum 9%, M. hominis 8.6% and M. genitalium 0.6%. The proportion of foreign patients positive for U. parvum was significantly higher compared to Italian patients (37% vs 30.1%, p = 0.007) and also for overall mycoplasma colonization (53.4% vs 45.8%, p = 0.011). The number of symptomatic patients positive for M. hominis was significantly higher than that of negative controls (2.9% vs 1%, p = 0.036). A significant positive trend in mycoplasma colonization was found in relation to the pregnancy week for U. urealyticum (p = 0.015), M. hominis (p = 0.044) and for overall mycoplasma colonization (p = 0.002).CONCLUSION:
multiplex RT-PCR can be a valuable tool to evaluate the real epidemiology of cervical mycoplasma colonization.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Ureaplasma
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Colo do Útero
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Ureaplasma urealyticum
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Infecções por Ureaplasma
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Mycoplasma hominis
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Mycoplasma genitalium
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Infecções por Mycoplasma
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article