Imipenem-resistant Gram-negative bacterial isolates carried by persons upon medical examination in Korea.
J Microbiol
; 55(8): 612-618, 2017 Aug.
Article
em En
| MEDLINE
| ID: mdl-28721477
ABSTRACT
Carbapenem-resistant Gram-negative bacteria (CR-GNB) have emerged and disseminated worldwide, become a great concern worldwide including Korea. The prevalence of fecal carriage of imipenem-resistant Gram-negative bacteria (IR-GNB) in persons in Korea was investigated. Stool samples were collected from 300 persons upon medical examination. Samples were screened for IR-GNB by using MacConkey agar with 2 µl/ml imipenem. Species were identified by 16S rRNA gene sequence analysis, and antimicrobial susceptibility was determined by the broth microdilution method. In total, 82 IR-GNB bacterial isolates were obtained from 79 (26.3%) out of 300 healthy persons. Multilocus sequence typing analysis showed very high diversity among IR P. aeruginosa, S. maltophilia, and E. cloacae isolates, and pulsed-field gel electrophoresis revealed five main pulsotypes of IR P. mirabilis. As for the presence of metallo-ß-lactamases (MBLs), only one IMP-25-producing S. marcescens isolate was identified. Although only one carbapenemase-producing isolate was identified, the high colonization rates with IR-GNB isolates in this study is notable because carriers may be a reservoir for the dissemination of resistant pathogens within the community as well as in health care institutions.
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Base de dados:
MEDLINE
Assunto principal:
Portador Sadio
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Imipenem
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Infecções por Bactérias Gram-Negativas
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Resistência beta-Lactâmica
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Bactérias Gram-Negativas
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Antibacterianos
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article