Preclinical pharmacokinetics and biodistribution studies of asenapine maleate using novel and sensitive RP-HPLC method.
Bioanalysis
; 9(14): 1037-1047, 2017 Jul.
Article
em En
| MEDLINE
| ID: mdl-28737445
ABSTRACT
AIM:
Asenapine maleate (ASPM) is a newer antipsychotic drug available as a sublingual tablet in the market. EXPERIMENTAL To investigate the pharmacokinetic and tissue distribution study of ASPM following oral administration in rats, reversed-phase HPLC method was developed and validated.RESULTS:
ASPM was extracted from plasma and tissue matrix by liquid-liquid extraction technique and analyzed using mobile phase consisted of phosphate buffer pH 3.0 and acetonitrile (6535% v/v). The method showed good linearity (10-500 ng/ml) with recovery 83-102%. In pharmacokinetics study, half-life was 32.74 ± 7.51 h due to slow elimination of drug. The biodistribution study indicated preferential distribution of ASPM to highly perfused organs.CONCLUSION:
The current method can be successfully applied for estimating the drug in various biological matrices.Palavras-chave
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MEDLINE
Assunto principal:
Cromatografia Líquida de Alta Pressão
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Cromatografia de Fase Reversa
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Limite de Detecção
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Compostos Heterocíclicos de 4 ou mais Anéis
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article