Characterization of Two Historic Smallpox Specimens from a Czech Museum.
Viruses
; 9(8)2017 07 27.
Article
em En
| MEDLINE
| ID: mdl-28749451
ABSTRACT
Although smallpox has been known for centuries, the oldest available variola virus strains were isolated in the early 1940s. At that time, large regions of the world were already smallpox-free. Therefore, genetic information of these strains can represent only the very last fraction of a long evolutionary process. Based on the genomes of 48 strains, two clades are differentiated Clade 1 includes variants of variola major, and clade 2 includes West African and variola minor (Alastrim) strains. Recently, the genome of an almost 400-year-old Lithuanian mummy was determined, which fell basal to all currently sequenced strains of variola virus on phylogenetic trees. Here, we determined two complete variola virus genomes from human tissues kept in a museum in Prague dating back 60 and 160 years, respectively. Moreover, mass spectrometry-based proteomic, chemical, and microscopic examinations were performed. The 60-year-old specimen was most likely an importation from India, a country with endemic smallpox at that time. The genome of the 160-year-old specimen is related to clade 2 West African and variola minor strains. This sequence likely represents a new endemic European variant of variola virus circulating in the midst of the 19th century in Europe.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Vírus da Varíola
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Varíola
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Genoma Viral
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Museus
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article