Nanoporous Immunoprotective Device for Stem-Cell-Derived ß-Cell Replacement Therapy.
ACS Nano
; 11(8): 7747-7757, 2017 08 22.
Article
em En
| MEDLINE
| ID: mdl-28763191
ABSTRACT
Encapsulation of human embryonic stem-cell-differentiated beta cell clusters (hES-ßC) holds great promise for cell replacement therapy for the treatment of diabetics without the need for chronic systemic immune suppression. Here, we demonstrate a nanoporous immunoprotective polymer thin film cell encapsulation device that can exclude immune molecules while allowing exchange of oxygen and nutrients necessary for in vitro and in vivo stem cell viability and function. Biocompatibility studies show the device promotes neovascular formation with limited foreign body response in vivo. The device also successfully prevented teratoma escape into the peritoneal cavity of mice. Long-term animal studies demonstrate evidence of engraftment, viability, and function of cells encapsulated in the device after 6 months. Finally, in vivo study confirms that the device was able to effectively immuno-isolate cells from the host immune system.
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MEDLINE
Assunto principal:
Nanotecnologia
/
Terapia Baseada em Transplante de Células e Tecidos
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article