A revised method for the preparation of dry bone samples used in histological examination: Five simple steps.

ABSTRACT

Histology of dry bone tissue has many scientific applications. The histological analysis of bone requires the production of good quality thin sections. Many researchers have developed new histological techniques and/or they have refined existing ones. In this paper, we describe a revision of histological techniques for obtaining thin sections from modern dry bone. The method is easy to apply and the equipment required is commonly found in a histology laboratory. In comparison to other techniques presented in the literature, this adapted method reduces the number of consumables and steps, thereby improving the efficiency and cost-effectiveness of the procedure.