Facile functionalization of peptide nucleic acids (PNAs) for antisense and single nucleotide polymorphism detection.
Org Biomol Chem
; 15(32): 6710-6714, 2017 Aug 16.
Article
em En
| MEDLINE
| ID: mdl-28766647
ABSTRACT
In this report, we show how a convenient on-resin copper-click functionalization of azido-functionalized peptide nucleic acids (PNAs) allows various PNA-based detection strategies. Firstly, a thiazole orange (TO) clicked PNA probe facilitates a binary readout when combined with F/Q labeled DNA, giving increased sensitivity for antisense detection. Secondly, our TO-PNA conjugate also allows single nucleotide polymorphism detection. Since antisense detection is also possible in the absence of the TO label, our sensing platform based on azido-d-ornithine containing PNA even allows for additional and more advanced functionalization and sensing strategies.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sondas Moleculares
/
DNA Antissenso
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Ácidos Nucleicos Peptídicos
/
Polimorfismo de Nucleotídeo Único
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article