Phase I and phase II metabolite identification of rutaecarpine in freshly isolated hepatocytes from male Sprague-Dawley rats.
Arch Pharm Res
; 40(8): 972-979, 2017 Aug.
Article
em En
| MEDLINE
| ID: mdl-28799086
Rutaecarpine, an alkaloid originally isolated from Evodia rutaecarpa, has been used for the treatment of gastrointestinal disorders in Asia. In the present study, the phase I and phase II metabolites of rutaecarpine were investigated in freshly isolated hepatocytes from male Sprague-Dawley rats. The individual metabolites were characterized via liquid chromatography-tandem mass spectrometry. The incubation of rutaecarpine with freshly isolated hepatocytes for 2 h yielded five major phase I metabolites. In addition, three glucuronide conjugates and four sulfate conjugates were observed. Because the majority of metabolites observed in vivo were identified, freshly isolated hepatocytes might be useful for the identification of certain metabolites formed from drug candidates from a reduced number of experimental animals.
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Base de dados:
MEDLINE
Assunto principal:
Quinazolinas
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Glucuronídeos
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Hepatócitos
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Alcaloides Indólicos
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Evodia
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article