Unveiling Cerebral Leishmaniasis: parasites and brain inflammation in Leishmania donovani infected mice.
Sci Rep
; 7(1): 8454, 2017 08 16.
Article
em En
| MEDLINE
| ID: mdl-28814754
ABSTRACT
Visceral leishmaniasis (VL) is a systemic disease with multifaceted clinical manifestations, including neurological signs, however, the involvement of the nervous system during VL is underestimated. Accordingly, we investigated both brain infection and inflammation in a mouse model of VL. Using bioluminescent Leishmania donovani and real-time 2D-3D imaging tools, we strikingly detected live parasites in the brain, where we observed a compartmentalized dual-phased inflammation pattern an early phase during the first two weeks post-infection, with the prompt arrival of neutrophils and Ly6Chigh macrophages in an environment presenting a variety of pro-inflammatory mediators (IFN-γ, IL-1ß, CXCL-10/CXCR-3, CCL-7/CCR-2), but with an intense anti-inflammatory response, led by IL-10; and a re-inflammation phase three months later, extremely pro-inflammatory, with novel upregulation of mediators, including IL-1ß, TNF-α and MMP-9. These new data give support and corroborate previous studies connecting human and canine VL with neuroinflammation and blood-brain barrier disruption, and conclusively place the brain among the organs affected by this parasite. Altogether, our results provide convincing evidences that Leishmania donovani indeed infects and inflames the brain.
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Base de dados:
MEDLINE
Assunto principal:
Leishmania donovani
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Leishmaniose
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Infecções Protozoárias do Sistema Nervoso Central
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Encefalite
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article