Uniform-sized neurosphere-mediated motoneuron differentiation in microwell arrays.
Electrophoresis
; 38(24): 3161-3167, 2017 12.
Article
em En
| MEDLINE
| ID: mdl-28815632
ABSTRACT
We developed the photocrosslinkable hydrogel microwell arrays for uniform-sized neurosphere-mediated motoneuron differentiation. Neural stem cells (NSCs) were obtained from embryonic cerebral cortex and spinal cord. To generate uniform-sized neurospheres in a homogeneous manner, the dissociated cells were cultured in the hydrogel microwell arrays for 3 days. Uniform-sized neurospheres harvested from microwell arrays were replated into laminin-coated substrate. In parallel, uniform-sized neurospheres cultured in microwell arrays were encapsulated by photocrosslinkable gelatin methacrylate hydrogels in a three-dimensional manner. We demonstrated the effect of hydrogel microwell sizes (e.g., 50, 100, 150 µm in diameter) on motoneuron differentiation, showing that the largest uniform-sized neurospheres derived from embryonic spinal cord efficiently differentiated into motoneurons. Therefore, this hydrogel microwell array could be a powerful array to regulate the uniform-sized neurosphere-mediated motoneuron differentiation.
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Base de dados:
MEDLINE
Assunto principal:
Diferenciação Celular
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Análise Serial de Tecidos
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Células-Tronco Neurais
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Neurônios Motores
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article