Microscale Laminar Vortices for High-Purity Extraction and Release of Circulating Tumor Cells.
Methods Mol Biol
; 1634: 65-79, 2017.
Article
em En
| MEDLINE
| ID: mdl-28819841
ABSTRACT
Circulating tumor cells (CTCs) are disseminated tumor cells that reflect the tumors of origin and can provide a liquid biopsy that would potentially enable noninvasive tumor profiling, treatment monitoring, and identification of targeted treatments. Accurate and rapid purification of CTCs holds great potential to improve cancer care but the task remains technically challenging. Microfluidic isolation of CTCs within microscale vortices enables high-throughput and size-based purification of rare CTCs from bodily fluids. Collected cells are highly pure, viable, and easily accessible, allowing seamless integration with various downstream applications. Here, we describe how to fabricate the High-Throughput Vortex Chip (Vortex-HT) and to process diluted whole blood for CTC collection. Lastly, immunostaining and imaging protocols for CTC classification and corresponding CTC image galleries are reported.
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MEDLINE
Assunto principal:
Separação Celular
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Técnicas Analíticas Microfluídicas
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Desenho de Equipamento
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Dispositivos Lab-On-A-Chip
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Células Neoplásicas Circulantes
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Neoplasias
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article